How To Install racon on Debian 12

Learn how to install racon on Debian 12 with this tutorial. racon is consensus module for raw de novo DNA assembly of long uncorrected reads

Introduction

In this tutorial we learn how to install racon on Debian 12.

What is racon

racon is:

Racon is intended as a standalone consensus module to correct raw contigs generated by rapid assembly methods which do not include a consensus step. The goal of Racon is to generate genomic consensus which is of similar or better quality compared to the output generated by assembly methods which employ both error correction and consensus steps, while providing a speedup of several times compared to those methods. It supports data produced by both Pacific Biosciences and Oxford Nanopore Technologies.

Racon can be used as a polishing tool after the assembly with either Illumina data or data produced by third generation of sequencing. The type of data inputed is automatically detected.

Racon takes as input only three files: contigs in FASTA/FASTQ format, reads in FASTA/FASTQ format and overlaps/alignments between the reads and the contigs in MHAP/PAF/SAM format. Output is a set of polished contigs in FASTA format printed to stdout. All input files can be compressed with gzip.

Racon can also be used as a read error-correction tool. In this scenario, the MHAP/PAF/SAM file needs to contain pairwise overlaps between reads including dual overlaps.

A wrapper script is also available to enable easier usage to the end- user for large datasets. It has the same interface as racon but adds two additional features from the outside. Sequences can be subsampled to decrease the total execution time (accuracy might be lower) while target sequences can be split into smaller chunks and run sequentially to decrease memory consumption. Both features can be run at the same time as well.

There are three methods to install racon on Debian 12. We can use apt-get, apt and aptitude. In the following sections we will describe each method. You can choose one of them.

Install racon Using apt-get

Update apt database with apt-get using the following command.

sudo apt-get update

After updating apt database, We can install racon using apt-get by running the following command:

sudo apt-get -y install racon

Install racon Using apt

Update apt database with apt using the following command.

sudo apt update

After updating apt database, We can install racon using apt by running the following command:

sudo apt -y install racon

Install racon Using aptitude

If you want to follow this method, you might need to install aptitude first since aptitude is usually not installed by default on Debian. Update apt database with aptitude using the following command.

sudo aptitude update

After updating apt database, We can install racon using aptitude by running the following command:

sudo aptitude -y install racon

How To Uninstall racon on Debian 12

To uninstall only the racon package we can use the following command:

sudo apt-get remove racon

Uninstall racon And Its Dependencies

To uninstall racon and its dependencies that are no longer needed by Debian 12, we can use the command below:

sudo apt-get -y autoremove racon

Remove racon Configurations and Data

To remove racon configuration and data from Debian 12 we can use the following command:

sudo apt-get -y purge racon

Remove racon configuration, data, and all of its dependencies

We can use the following command to remove racon configurations, data and all of its dependencies, we can use the following command:

sudo apt-get -y autoremove --purge racon

Dependencies

racon have the following dependencies:

References

Summary

In this tutorial we learn how to install racon package on Debian 12 using different package management tools: apt, apt-get and aptitude.